主题

铁离子结合

内容:铁离子结合是一个研究课题。betway亚洲在整个生命周期中,该主题共发表了42篇论文,获得了379次引用。
论文
多个过滤器

期刊文章 DOI
07年11月2007 - BMC基因组学
TL;博士:识别雄甾酮生物合成和代谢的新途径和基因是寻找育种目的分子标记的重要的第一步,有助于理解控制和响应猪睾丸中雄甾酮水平的复杂遗传系统。
文摘:当使用未阉割的公猪进行猪生产时,野猪污染是一个主要障碍。造成这种污染的主要化合物之一是雄烯酮,这是猪睾丸中产生的一种信息素。本研究利用微阵列技术研究了高雄烯酮和低雄烯酮公猪睾丸中数千个基因的同时表达。该研究允许识别与雄烯酮水平升高相关的基因和途径,这对于识别潜在的分子标记以进行育种至关重要。收集了60只公猪的睾丸组织,其中30只雄烯酮水平极高,30只雄烯酮水平极低,分别来自杜洛克和挪威长白猪两个品种。将样本与包含26877个cDNA克隆的猪阵列杂交,检测到杜洛克和挪威长白猪中差异表达基因分别为563个和160个(p < 0.01)。在这些显著上调和下调的克隆中,有72个在两个品种中是常见的,这表明在公猪雄甾酮水平的调节或反应中可能存在一般和品种特异性机制。选择10个基因进行real竞争性PCR和real-time PCR的表达模式验证。正如预期的那样,我们的研究结果表明,类固醇激素代谢和生物合成是雄甾酮水平的重要生物过程,但也发现了其他潜在的途径。其中氧化还原酶活性、铁结合、铁离子结合和电子传递活性。 Genes belonging to the cytochrome P450 and hydroxysteroid dehydrogenase families were highly up-regulated, in addition to several genes encoding different families of conjugation enzymes. Furthermore, a number of genes encoding transcription factors were found both up- and down-regulated. The high number of clones belonging to ferric iron and iron ion binding suggests an importance of these genes, and the association between these pathways and androstenone levels is not previously described. This study contributes to the understanding of the complex genetic system controlling and responding to androstenone levels in pig testis. The identification of new pathways and genes involved in the biosynthesis and metabolism of androstenone is an important first step towards finding molecular markers to reduce boar taint.

55引用


期刊文章 DOI
TL;博士:RNA和蛋白质毒性机制的重叠被认为,提供了对RNA在polyQ疾病中的致病性的见解。
文摘:脊髓小脑性共济失调3型是多谷氨酰胺(polyQ)疾病的一种,是由相关基因编码区内的caga重复扩增引起的。CAG重复指定谷氨酰胺,扩展的polyQ结构域突变赋予蛋白质显性毒性。传统上,研究集中在多q疾病的蛋白质毒性机制。然而,最近的研究表明,编码有毒polyQ蛋白的cagr -repeat RNA也导致了果蝇的这种疾病。为了深入了解RNA毒性的本质,我们从表达有毒的CAA -repeat mRNA (CAG100)和无毒的中断CAA/G mRNA repeat (CAA/G105)的苍蝇中提取了脑富集RNA,用于微阵列分析。这种方法确定了160个基因,它们在CAG100果蝇中特异表达。功能注释聚类分析揭示了CAG100基因表中丰富的几个广泛的本体,包括铁离子结合和核苷酸结合。有趣的是,Hsp70基因(polyQ和其他人类神经退行性疾病的强大抑制因子)的转录本也上调了。因此,我们进行了测试,并表明热休克蛋白70的上调可以减轻CAG-repeat RNA的毒性。然后我们评估了致病性的扩展Ataxin-3 polyQ蛋白的其他修饰物是否也可以修饰CAG-repeat RNA毒性。 This approach identified the co-chaperone Tpr2, the transcriptional regulator Dpld, and the RNA-binding protein Orb2 as modifiers of both polyQ protein toxicity and CAG-repeat RNA-based toxicity. These findings suggest an overlap in the mechanisms of RNA and protein-based toxicity, providing insights into the pathogenicity of the RNA in polyQ disease.

48引用


期刊文章 DOI
Rafaqat A. Gill 1Basharat阿里 2Basharat阿里 1彭崔 1 + 6更 机构(3.
07年11月2016 - BMC基因组学
TL;博士:RNA-Seq法通过激活植物代谢、胁迫响应基因、转录因子和蛋白质百科等方法,探索了Cr的危害行为和GSH的上调机制,结果表明,品种ZS 758具有较好的代谢能力,对Cr毒性具有较高的耐受性。
文摘:铬(Cr)是多种工业使用的元素,被认为是一种潜在的环境威胁。铬被发现是一种潜在的水和土壤污染物,因此是目前关注的领域。油菜(甘蓝型油菜)是全球著名的食用油主要来源。甘蓝型油菜生长速度快,生物量大,吸收有毒物质能力强,被认为是一种潜在的抗病候选植物。到目前为止,还没有研究在RNA-Seq水平上描述Cr和GSH的机制。数字基因表达(DGE)和转录组谱分析(TPA)方法为揭示甘蓝型油菜两个良种(zs758和浙大622)Cr胁迫和GSH缓解机制的相关基因提供了新的思路。数据显示,Cr抑制KEGG通路,即二苯乙烯类、二芳七酮类和姜辣酚的生物合成;ZS 758对柠檬烯、戊糖降解及谷胱甘肽代谢的影响以及浙大622的核糖体和硫代葡萄糖苷的生物合成。zs758诱导了维生素B6、色氨酸、硫、氮、果糖和甘糖的代谢;泽大622诱导了玉米素的生物合成、亚油酸的代谢、精氨酸和脯氨酸的代谢以及丙氨酸、天冬氨酸和谷氨酸的代谢途径。 Cr increased the TFs that were related to hydralase activity, antioxidant activity, catalytic activity phosphatase and pyrophosphatase activity in ZS 758, and vitamin binding and oxidoreductase activity in Zheda 622. Cr also up-regulated the promising proteins related to intracellular membrane bounded organelles, nitrile hyrdatase activity, cytoskeleton protein binding and stress response. It also uncovered, a novel Cr-responsive protein (CL2535.Contig1_All) that was statistically increased as compared to control and GSH treated plants. Exogenously applied GSH successfully not only recovered the changes in metabolic pathways but also induced cysteine and methionine metabolism in ZS 758 and ubiquinone and other terpenoid-quinone biosynthesis pathways in Zheda 622. Furthermore, GSH increased the level of TFs i.e. the gene expression of antioxidant and catalytic activities, iron ion binding and hydrolase activity as compared with Cr. Moreover, results pointed out a novel GSH responsive protein (CL827.Contig3_All) whose expression was found to be significantly increased when compared than Cr stress. Results further delineated that GSH induced TFs such as glutathione disulphide oxidoreducatse and aminoacyl-tRNA ligase activity, and beta glucosidase activity in ZS 758. Similarly in Zheda 622, GSH induced the TFs for instance DNA binding and protein dimerization activity. GSH also highlighted the proteins that were involved in transportation, photosynthesis process, RNA polymerase activity, and against the metal toxicity. These results indicated that cultivar ZS 758 had better metabolism and showed higher tolerance against Cr toxicity. The responses of ZS 758 and Zheda 622 differed considerably at both physiological and transcriptional level. Moreover, RNA-Seq method explored the hazardous behavior of Cr as well as GSH up-regulating mechanism by activating plant metabolism, stress responsive genes, TFs and protein encyclopedia.

46引用


期刊文章 DOI
余张 1Zheyu李 1谢尔盖Kholodkevich 2沙洛夫 2 + 3更 机构(2
TL;博士:结果表明,肝胰脏积累Cd具有时间和剂量依赖性,且DEGs分别与氧化还原过程、免疫系统、离子稳态、消化吸收和atp酶有关。
文摘:镉(Cd)是环境中常见的污染物。小龙虾被认为适合用于指示重金属对环境的影响。然而,关于接触Cd对克氏原螯虾肝胰脏造成损伤的机制的信息有限。我们将成年雄性克氏原螯虾暴露在2.0、5.0和10.0 mg/L Cd下24、48和72 h,以探讨Cd的毒性。随后,我们测量肝胰脏中的生物积累,并测定丙二醛(MDA)、超氧化物歧化酶(SOD)、过氧化氢酶(CAT)和谷胱甘肽s -转移酶(GST)。并对Cd胁迫下的克氏P. clarkii肝胰脏进行组织病理学分析和转录组分析。结果表明,肝胰脏能够以时间和剂量依赖性的方式积累Cd。Cd诱导MDA含量和抗氧化酶活性发生显著变化。小龙虾肝胰脏发生了严重的组织学改变。在2.0、5.0和10.0 mg/L Cd中暴露72 h后,转录组分析分别鉴定出1061、747和1086个差异表达基因(DEGs)。5.0 mg/L Cd抑制血红素结合、四吡啶结合、铁离子结合及氧化还原酶和硫转移酶活性,10.0 mg/L Cd增强核物质输出。 In the hepatopancreas treated with 10.0 mg/L Cd, pathways related to diseases and immune system were significantly enriched. Meanwhile, 31, 31, 24, 7, and 12 identified DEGs were associated with the oxidation-reduction process, immune system, ion homeostasis, digestion and absorption, and ATPases, respectively. Our study provides comprehensive information for exploring the toxic mechanisms of Cd and candidate biomarkers for aquatic Cd risk evaluation.

35引用


期刊文章 DOI
11月19日2019 - Mbio
TL;博士:研究结果支持了基因调节变化在褐腐菌适应中发挥关键作用的假设,并揭示了这些独特真菌之间的重要生物学变化,但它们也可能阐明了限制褐腐菌在特定生态位中的一个特征。
文摘:真菌在木质生物质中碳的回收中占主导地位。这种有机转化过程首先在“白腐”真菌中进化,白腐真菌降解木质素以获取碳水化合物,后来又多次进化为更有效的策略,选择性地针对碳水化合物——“棕腐”。棕腐真菌的适应性通常可以用部署活性氧(ROS)以氧化攻击木结构的机制来解释。然而,其遗传基础尚不清楚,特别是在传统碳水化合物活性酶(CAZYs)相对于白腐病祖先的基因收缩的背景下。在这里,我们假设,尽管基因丢失,但这些褐腐病效率的明显提高部分是由于保留基因的上调。我们将比较转录组学应用于生长在木晶片上的两种腐烂类型的多个物种,以创建渐进腐烂的梯度,并跟踪时间基因表达。数十个“腐烂阶段依赖性”的基因被分离出来,缩小了褐腐真菌特有的具有时间依赖性调控的候选基因库。CAZY家族表达时间的广泛比较表明,与白腐菌相比,木质纤维素氧化基因在褐腐菌中向早期阶段的时间调节转移,使水解前的氧化处理分离成为可能。分离到具有铁离子结合功能的褐腐ros生成关键基因。此外,转录能量转移到褐腐真菌中残留的GHs上,以加强碳水化合物的转化。综上所述,这些结果支持了基因调节的改变在褐腐病的适应中起关键作用的假说。 IMPORTANCE Fungi dominate the turnover of wood, Earth’s largest pool of aboveground terrestrial carbon. Fungi first evolved this capacity by degrading lignin to access and hydrolyze embedded carbohydrates (white rot). Multiple lineages, however, adapted faster reactive oxygen species (ROS) pretreatments to loosen lignocellulose and selectively extract sugars (brown rot). This brown rot “shortcut” often coincided with losses (>60%) of conventional lignocellulolytic genes, implying that ROS adaptations supplanted conventional pathways. We used comparative transcriptomics to further pursue brown rot adaptations, which illuminated the clear temporal expression shift of ROS genes, as well as the shift toward synthesizing more GHs in brown rot relative to white rot. These imply that gene regulatory shifts, not simply ROS innovations, were key to brown rot fungal evolution. These results not only reveal an important biological shift among these unique fungi, but they may also illuminate a trait that restricts brown rot fungi to certain ecological niches.

24引用


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